Despite repeated round of negotiations, there is increasing doubts on whether nuclear talks between the two countries will lead to an agreement.
Iran's Supreme Leader Ayatollah Ali Khamenei has said U.S. demands for Tehran to stop enriching uranium are "excessive and outrageous".
His comments, reported by state media, cast further doubt on the likelihood of progress in the ongoing nuclear negotiations.
The main point of contention continues to be Iran’s right to enrich uranium, a process the U.S. views as a potential pathway to nuclear weapons.
U.S. special envoy to the Middle East Steve Witkoff says the Trump administration’s “red line” in nuclear talks with Iran is that Tehran cannot maintain any ability to enrich uranium.
But Iranian Deputy Foreign Minister Majid Takht-Ravanchi warned that negotiations would collapse if Washington insists on this condition.
Tehran maintains that its nuclear programme is solely for peaceful energy purposes.
Iran continues to be under pressure from Washington, with President Donald Trump last week cautioning that “something bad” could happen if Iran did not act quickly.
The future of the talks between the two countries remains uncertain, despite earlier indications that a fifth round could be held in Rome this weekend.
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